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March20 podcast Dr Hazen
anti-TMA pill in a year or 2 ? (scroll 12 mins)

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MEBO - UBIOME study 2018



MEBO Gut Microbiome Study
"Microbial Basis of Systemic Malodor and PATM Conditions (PATM)"
Funded by uBiome Research Grant

"Microbial Basis of Systemic Malodor and PATM Conditions (PATM)"

Dynamics of the Gut Microbiota in
Idiopathic Malodor Production

Started May 2018 - Ongoing

Current people sent kits : 100/100
3 kits per person


Participation info : LINK English

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BO Sufferers Podcasts



TMAU Petition world
TMAU UK end total:262
TMAU UK ends 23/01/20
TMAU Petition USA end total 204
USA : Moveon open
TMAU (Dominican)
Metabolomic Profiling Study

Start : Aug 2016
Stage 1 : 27 Canadian volunteers to test
Latest click here (26 oct) :
17 samples returned

Note : Stage 1 is Canada only.
Return cut-off date : passed
Analysis can take 6/8 weeks
Analysis start in/before Nov
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NORD Member Organization
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Denver TMAU Test Lab survey click here
click to Read more/less

USA survey for anyone who wants to improve Denver TMAU test

begun : Dec22
end : no ending for now

A trainee genetic counselor is working at the Denver TMAU test lab. Probably as part of her training. As a project she wishes feedback on any aspect of the Denver TMAU test and process. You can fill in the survey and/or email her (email address is in survey). It's meant for USA people, but perhaps others can give their view too (as we have so few opportunities).

quote from her rareconnect post

"Hello all! I wanted to make you aware of a research study being conducted to better understand the experience and needs of individuals with trimethylaminuria with a goal of being able to create improved patient and healthcare provider education materials. Any participation is completely voluntary and all responses remain confidential. Feel free to use the contact information within the link with any questions or share the survey with others with TMAU."

see this post for more details

Saturday, September 4, 2010

New HBRI FMO research paper

Dr John Cashman of the Human Biomolecular Research Institute in San Diego is probably the most prolific researcher (of the few researching) of the FMO group of enzymes. This month he has published another research paper. It looks as if it is to do with FMO enzyme sources for research labs, so likely has no direct connection with TMAU other than it may make research on the FMO enzymes easier in the future.

Oligomerization and kinetic characterization of human FMO3 and FMO5 expressed as maltose binding protein fusions.
Reddy RR, Ralph EC, Motika MS, Zhang J, Cashman JR.

1 Human Biomolecular Research Institute;
The flavin-containing monooxygenase (FMO) family of enzymes oxygenates nucleophilic xenobiotics and endogenous substances. Human FMO3 and FMO5 are the predominant FMO forms in adult liver. These enzymes are naturally membrane-bound, and recombinant proteins are commercially available as microsomal preparations from insect cells (i.e., Supersome FMO). Alternatively, FMO3 has previously been expressed as a soluble protein, through use of an N-terminal maltose binding protein (MBP) fusion. In the current study, MBP fusions of both human FMO3 and FMO5 were prepared to >90% purity in the presence of detergent, characterized for biochemical and kinetic parameters, and the parameters were compared to those of Supersome FMO samples. Although MBP-FMO enzymes afforded lower rates of turnover compared with the corresponding Supersome FMOs, both types of FMO showed identical substrate dependencies and similar responses to changes in assay conditions. Interestingly, the FMO3 enzymes showed a 2-fold activation of k(cat)/K(m) n the presence of Triton X-100. Oligomeric analysis of MBP-FMO3 also showed disassociation from a high-order oligomeric form to a monomeric status in the presence of Triton X-100. This report serves as the first direct comparison between Supersome FMOs and the corresponding MBP-fusions, and the first report of a detergent-based activation of k(cat)/K(m) that corresponds to changes in oligomerization.
The full paper can be read for free here:

One interesting note was that FMO5 seems to be quite abundant in the small intestine. It does not seem to be known what role FMO5 plays, although often people with TMAU feel they have mild 'gut issues'. Perhaps FMO5 plays a detoxifying role in the gut, without which the ecology tends to get unfavorably 'out of control'

Update : It now seems it is unlikely FMO5 has an important role in humans. It seems to be very 'substrate specific' and deals with very few substrates, and although the RNA may be found in adult human cells, this does not mean it turns into FMO5 protein.


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